Blend Taq -Plus-

Cosmo Bio USA

Blend Taq -Plus-

16

Research Use Only

For PCR Blend Taq® and Blend Taq® -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns method(1). This method uses a DNA polymerase which lacked 3 to 5 exonuclease (proofreading) activity (e.g., TaqDNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a mixed enzyme solution enables highly efficient amplification. Blend Taq® and Blend Taq® -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method. Application: PCR Features: Effective for the amplification of various targets from small starting template amounts. The use of Hot Start technology with anti-Taq DNA polymerase antibodies results in highly efficient amplification. <Blend Taq® (Code No. TYB-BTQ-101) does not use hot start> The PCR error ratio of this enzyme is approximately 3-4 times less than that of Taq DNA polymerase.

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