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ProductsBack/BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI) Polyclonal Antibody

BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI) Polyclonal Antibody

CAU21699
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetBAMBI
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Overview

  • Supplier
    Biomatik
  • Product Name
    BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID25805
  • Target name
    BAMBI
  • Target description
    BMP and activin membrane bound inhibitor
  • Target synonyms
    NMA, BMP and activin membrane-bound inhibitor homolog, non-metastatic gene A protein, putative transmembrane protein NMA
  • Host
    Rabbit
  • Protein IDQ13145
  • Protein Name
    BMP and activin membrane-bound inhibitor homolog
  • Scientific Description
    The BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BAMBI using anti-BAMBI antibody (A07983-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAMBI antigen affinity purified polyclonal antibody (Catalog # A07983-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAMBI at approximately 29KD. The expected band size for BAMBI is at 29KD.
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