Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BMP4 Polyclonal Antibody, Unconjugated (bs-1374R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BMP4 Polyclonal Antibody, Unconjugated (bs-1374R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
BMP4 Polyclonal Antibody
BS-1374R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Sheep
Overview
- SupplierBioss
- Product NameBMP4 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityHuman, Mouse, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
References
- Yamaji K, Morita J, Watanabe T, et al. Maldevelopment of the submandibular gland in a mouse model of apert syndrome. Dev Dyn. 2018,247(11):1175-1185. doi: 10.1002/dvdy.24673Read this paper