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Western Blot analysis of 4T1 cells using BMP-6 Polyclonal Antibody
Western Blot analysis of 4T1 cells using BMP-6 Polyclonal Antibody
Western Blot analysis of 4T1 cells using BMP-6 Polyclonal Antibody

BMP6 Antibody

CSB-PA006335
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBMP6
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Overview

  • Supplier
    Cusabio
  • Product Name
    BMP6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID654
  • Target name
    BMP6
  • Target description
    bone morphogenetic protein 6
  • Target synonyms
    bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); VG-1-R; VG-1-related protein; VGR; VGR1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22004
  • Protein Name
    Bone morphogenetic protein 6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BMP6 using anti-BMP6 antibody (PB9690). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP6 antigen affinity purified polyclonal antibody (Catalog # PB9690) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP6 at approximately 43 kDa. The expected band size for BMP6 is at 57 kDa.
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