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Western Blot Positive WB detected in: Rat lung tissue All lanes: BMPR1B antibody at 2.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 61 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Rat lung tissue All lanes: BMPR1B antibody at 2.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 61 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Rat lung tissue All lanes: BMPR1B antibody at 2.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 61 kDa Observed band size: 57 kDa

BMPR1B Antibody

CSB-PA002751LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetBMPR1B
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Overview

  • Supplier
    Cusabio
  • Product Name
    BMPR1B Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID658
  • Target name
    BMPR1B
  • Target description
    bone morphogenetic protein receptor type 1B
  • Target synonyms
    ALK-6, ALK6, AMD3, AMDD, BDA1D, BDA2, CDw293, bone morphogenetic protein receptor type-1B, BMP type-1B receptor, BMPR-1B, activin receptor-like kinase 6, bone morphogenetic protein receptor, type IB, serine/threonine receptor kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00238
  • Protein Name
    Bone morphogenetic protein receptor type-1B
  • Scientific Description
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5. Positively regulates chondrocyte differentiation through GDF5 interaction.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BMPR1B using anti-BMPR1B antibody (PB9528). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMPR1B antigen affinity purified polyclonal antibody (Catalog # PB9528) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMPR1B at approximately 57 kDa. The expected band size for BMPR1B is at 57 kDa.
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