Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BNIP2 antibody (GTX114283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![BNIP2 antibody detects BNIP2 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP2 protein stained by BNIP2 antibody (GTX114283) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX114283/GTX114283_40702_20170824_IFA_w_23060501_243.webp "BNIP2 antibody detects BNIP2 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP2 protein stained by BNIP2 antibody (GTX114283) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BNIP2 antibody (GTX114283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
BNIP2 antibody
GTX114283
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetBNIP2
Overview
- SupplierGeneTex
- Product NameBNIP2 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.95 mg/ml
- ConjugateUnconjugated
- Gene ID663
- Target nameBNIP2
- Target descriptionBCL2 interacting protein 2
- Target synonymsBNIP-2, NIP2, BCL2/adenovirus E1B 19 kDa protein-interacting protein 2, BCL2/adenovirus E1B 19kDa interacting protein 2
- HostRabbit
- IsotypeIgG
- Protein IDQ12982
- Protein NameBCL2/adenovirus E1B 19 kDa protein-interacting protein 2
- Scientific DescriptionThis gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. Though the specific function is unknown, it interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. [provided by RefSeq]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Pan M, Chew TW, Wong DCP, et al. BNIP-2 retards breast cancer cell migration by coupling microtubule-mediated GEF-H1 and RhoA activation. Sci Adv. 2020,6(31):eaaz1534. doi: 10.1126/sciadv.aaz1534Read this paper
Datasheet
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