Immunohistochemistry of proBNP staining in Paraffin section of Human tibial growth plate. Antigen retrieval was performed in 5 ug/ml proteinase K in 100mM Tris pH 8.0, 50mM EDTA for 10min at 37°C. The sections were incubated with AM02178PU (1/25) and detected using the biotin-streptavidin method. DAB was used as substrate. Sections were counterstained with hematoxylin. Image B: AM02178PU stains the late proliferative, prehypertrophic chondrocytes but not the resting and early proliferating cells. Image C: AM02178PU preincubated with proBNP (aa 1-21) does not stain the section. Marchini et al. (2007). Human Molecular Genetics 16 (24): 3081-3087.