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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA437965(BOK(BH3 domain) Antibody) at dilution 1/20, on the right is treated with synthetic peptide.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA437965(BOK(BH3 domain) Antibody) at dilution 1/20, on the right is treated with synthetic peptide.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA437965(BOK(BH3 domain) Antibody) at dilution 1/20, on the right is treated with synthetic peptide.

BOK Antibody

CSB-PA437965
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBOK
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Overview

  • Supplier
    Cusabio
  • Product Name
    BOK Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID666
  • Target name
    BOK
  • Target description
    BCL2 family apoptosis regulator BOK
  • Target synonyms
    BCL2 related ovarian killer; BCL2L9; bcl2-L-9; bcl-2-like protein 9; bcl-2-related ovarian killer protein; BOK, BCL2 family apoptosis regulator; BOKL; hBOK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UMX3
  • Protein Name
    Bcl-2-related ovarian killer protein
  • Scientific Description
    The protein encoded by this gene belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BOK using anti-BOK antibody (A05577-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat ovary tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse ovary tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BOK antigen affinity purified polyclonal antibody (Catalog # A05577-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BOK at approximately 27 kDa. The expected band size for BOK is at 23 kDa.
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