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Bone Sialoprotein antibody

GTX44333
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
Product group Antibodies
ReactivityHuman
TargetIBSP
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Overview

  • Supplier
    GeneTex
  • Product Name
    Bone Sialoprotein antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3381
  • Target name
    IBSP
  • Target description
    integrin binding sialoprotein
  • Target synonyms
    BNSP, BSP, BSP-II, SP-II, integrin-binding sialoprotein, BSP II, bone sialoprotein 2, bone sialoprotein II, cell-binding sialoprotein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21815
  • Protein Name
    Integrin-binding sialoprotein
  • Scientific Description
    The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IBSP using anti-IBSP antibody (A03183). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IBSP antigen affinity purified polyclonal antibody (Catalog # A03183) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IBSP at approximately 65 kDa. The expected band size for IBSP is at 35 kDa.
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