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ProductsBack/Bone Sialoprotein (IBSP) (native) Mouse Monoclonal Antibody [Clone ID: ID1.2]
Immunoblot analysis of AM02057PU-N/AM02057PU-S specificty. Native and recombinant Bone proteins were separated by SDS-PAGE and immunoblotted with this BSP antibody Cat.-No AM02057PU-N/AM02057PU-S at 1/4000 dilution. This antibody did not detect BSP purified from Porcine Bone, nor Porcine Osteopontin (OPN) or SPARC/Osteonectin (ON). Gogan G et al. (2004) Connect Tissue Res 45 (1):60-71
Immunoblot analysis of AM02057PU-N/AM02057PU-S specificty. Native and recombinant Bone proteins were separated by SDS-PAGE and immunoblotted with this BSP antibody Cat.-No AM02057PU-N/AM02057PU-S at 1/4000 dilution. This antibody did not detect BSP purified from Porcine Bone, nor Porcine Osteopontin (OPN) or SPARC/Osteonectin (ON). Gogan G et al. (2004) Connect Tissue Res 45 (1):60-71
Immunoblot analysis of AM02057PU-N/AM02057PU-S specificty. Native and recombinant Bone proteins were separated by SDS-PAGE and immunoblotted with this BSP antibody Cat.-No AM02057PU-N/AM02057PU-S at 1/4000 dilution. This antibody did not detect BSP purified from Porcine Bone, nor Porcine Osteopontin (OPN) or SPARC/Osteonectin (ON). Gogan G et al. (2004) Connect Tissue Res 45 (1):60-71

Bone Sialoprotein (IBSP) (native) Mouse Monoclonal Antibody [Clone ID: ID1.2]

AM02057PU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, RadioImmunoAssay
Product group Antibodies
ReactivityHuman
TargetIBSP
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Bone Sialoprotein (IBSP) (native) Mouse Monoclonal Antibody [Clone ID: ID1.2]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ID1.2
  • Gene ID3381
  • Target name
    IBSP
  • Target description
    integrin binding sialoprotein
  • Target synonyms
    BNSP, BSP, BSP-II, SP-II, integrin-binding sialoprotein, BSP II, bone sialoprotein 2, bone sialoprotein II, cell-binding sialoprotein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP21815
  • Protein Name
    Integrin-binding sialoprotein
  • Scientific Description
    Bone Sialoprotein (IBSP) (native) mouse monoclonal antibody, clone ID1.2, Purified
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IBSP using anti-IBSP antibody (A03183). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IBSP antigen affinity purified polyclonal antibody (Catalog # A03183) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IBSP at approximately 65 kDa. The expected band size for IBSP is at 35 kDa.
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