ELISA plate was coated with serially diluted Bovine Type V Collagen (SB Cat. No. 1280-02S). Purified collagen was detected with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01), Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01), Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01), Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01), and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).