![WB analysis of Raji (1), and Jurkat (2) cell lysate using GTX60654 Brachyury antibody [1H9A2]. WB analysis of Raji (1), and Jurkat (2) cell lysate using GTX60654 Brachyury antibody [1H9A2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60654/GTX60654_20170912_WB_1_w_23061123_874.webp)
WB analysis of Raji (1), and Jurkat (2) cell lysate using GTX60654 Brachyury antibody [1H9A2].
Brachyury antibody [1H9A2]
GTX60654
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTBXT
Overview
- SupplierGeneTex
- Product NameBrachyury antibody [1H9A2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FCM: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1H9A2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6862
- Target nameTBXT
- Target descriptionT-box transcription factor T
- Target synonymsSAVA, T, TFT, T-box transcription factor T, T brachyury transcription factor, T, brachyury homolog, brachyury protein, protein T
- HostMouse
- IsotypeIgG1
- Protein IDO15178
- Protein NameT-box transcription factor T
- Scientific DescriptionThe protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![IHC-P analysis of cervical cancer tissue using GTX60654 Brachyury antibody [1H9A2]. IHC-P analysis of cervical cancer tissue using GTX60654 Brachyury antibody [1H9A2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60654/GTX60654_20170912_IHC-P_w_23061123_441.webp)
![WB analysis of HEK293 (1) and Brachyury (AA: 257-309)-hIgGFc transfected HEK293 (2) cell lysate using GTX60654 Brachyury antibody [1H9A2]. WB analysis of HEK293 (1) and Brachyury (AA: 257-309)-hIgGFc transfected HEK293 (2) cell lysate using GTX60654 Brachyury antibody [1H9A2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60654/GTX60654_20170912_WB_w_23061123_567.webp)
![ELISA analysis of antigen using GTX60654 Brachyury antibody [1H9A2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng ELISA analysis of antigen using GTX60654 Brachyury antibody [1H9A2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60654/GTX60654_20170912_ELISA_w_23061123_932.webp)
![IHC-P analysis of rectum cancer tissue using GTX60654 Brachyury antibody [1H9A2]. IHC-P analysis of rectum cancer tissue using GTX60654 Brachyury antibody [1H9A2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60654/GTX60654_20170912_IHC-P_1_w_23061123_639.webp)
![FACS analysis of HeLa cells using GTX60654 Brachyury antibody [1H9A2]. Green : Brachyury Red : negative control FACS analysis of HeLa cells using GTX60654 Brachyury antibody [1H9A2]. Green : Brachyury Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60654/GTX60654_20170912_FACS_w_23061123_333.webp)



![Brachyury antibody [HL2285] detects Brachyury protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brachyury stained by Brachyury antibody [HL2285] (GTX638338) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638338/GTX638338_45110_20231222_ICC_IF_R_24011618_641.webp)
![IHC-P analysis of human bone chordoma tissue using GTX639945 Brachyury antibody [HMV321] HistoMAX?. Chordoma with intense brachyury staining of all tumor cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639945/GTX639945_20240522_IHC-P_2_24052200_449.webp)
![ELISA analysis of antigen using GTX60616 Brachyury antibody [6C12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60616/GTX60616_20170912_ELISA_w_23061123_325.webp)

![Brachyury antibody detects Brachyury protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brachyury stained by Brachyury antibody (GTX131339) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX131339/GTX131339_44412_20220527_ICC_IF_22062919_492.webp)