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Bradykinin Polyclonal Antibody

BS-10947R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKNG1
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Overview

  • Supplier
    Bioss
  • Product Name
    Bradykinin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3827
  • Target name
    KNG1
  • Target description
    kininogen 1
  • Target synonyms
    BDK, BK, HAE6, HK, HMWK, KNG, kininogen-1, alpha-2-thiol proteinase inhibitor, bradykinin, fitzgerald factor, high molecular weight kininogen, williams-Fitzgerald-Flaujeac factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01042
  • Protein Name
    Kininogen-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kininogen 1/KNG1 using anti-Kininogen 1/KNG1 antibody (PB9278). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kininogen 1/KNG1 antigen affinity purified polyclonal antibody (Catalog # PB9278) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kininogen 1/KNG1 at approximately 72 kDa. The expected band size for Kininogen 1/KNG1 is at 72 kDa.
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