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Western Blot analysis of 293 cells using BRCA1 Polyclonal Antibody
Western Blot analysis of 293 cells using BRCA1 Polyclonal Antibody
Western Blot analysis of 293 cells using BRCA1 Polyclonal Antibody

BRCA1 Antibody

CSB-PA001041
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBRCA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    BRCA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID672
  • Target name
    BRCA1
  • Target description
    BRCA1 DNA repair associated
  • Target synonyms
    BRCA1/BRCA2-containing complex, subunit 1; BRCAI; BRCC1; breast and ovarian cancer susceptibility protein 1; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; BROVCA1; early onset breast cancer 1; Fanconi anemia, complementation group S; FANCS; IRIS; PNCA4; PPP1R53; protein phosphatase 1, regulatory subunit 53; PSCP; RING finger protein 53; RNF53
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP38398
  • Protein Name
    Breast cancer type 1 susceptibility protein
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BRCA1 using anti-BRCA1 antibody (A00005-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (Catalog # A00005-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCA1 at approximately 290 kDa. The expected band size for BRCA1 is at 210 kDa.
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