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BRD1 antibody detects BRD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. BRD1 stained by BRD1 antibody (GTX115758) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BRD1 antibody detects BRD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. BRD1 stained by BRD1 antibody (GTX115758) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BRD1 antibody detects BRD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. BRD1 stained by BRD1 antibody (GTX115758) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BRD1 antibody

GTX115758
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetBRD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    BRD1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.94 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23774
  • Target name
    BRD1
  • Target description
    bromodomain containing 1
  • Target synonyms
    BRL, BRPF1, BRPF2, bromodomain-containing protein 1, BR140-like protein, bromodomain and PHD finger-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95696
  • Protein Name
    Bromodomain-containing protein 1
  • Scientific Description
    This gene encodes a protein of unknown function. The protein contains a bromodomain, a sequence motif often found in transcriptional coactivators, and localizes to the nucleus in testis and several other cell types. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of BRD1 using anti-BRD1 antibody (A05302-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD1 antigen affinity purified polyclonal antibody (Catalog # A05302-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD1 at approximately 120 kDa. The expected band size for BRD1 is at 120 kDa.
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