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Lane 1: SiHa Cells; Probed with BRD2 (6B5) Monoclonal Antibody (bsm-54434R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: SiHa Cells; Probed with BRD2 (6B5) Monoclonal Antibody (bsm-54434R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: SiHa Cells; Probed with BRD2 (6B5) Monoclonal Antibody (bsm-54434R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BRD2 Recombinant Antibody

BSM-54434R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetBRD2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    BRD2 (6B5) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), Flow-Cyt(1ug/Test), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID6046
  • Target name
    BRD2
  • Target description
    bromodomain containing 2
  • Target synonyms
    BRD2 intronic transcript 1; BRD2-IT1; bromodomain-containing protein 2; D6S113E; female sterile homeotic-related gene 1; FSH; FSRG1; NAT; O27.1.1; really interesting new gene 3 protein; RING3; RNF3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25440
  • Protein Name
    Bromodomain-containing protein 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BRD2 using anti-BRD2 antibody (A02412-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD2 antigen affinity purified polyclonal antibody (Catalog # A02412-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD2 at approximately 110 kDa. The expected band size for BRD2 is at 88 kDa.
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