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ProductsBack/BRDG1 (F-12) Alexa Fluor(r) 647

BRDG1 (F-12) Alexa Fluor(r) 647

SC-398691AF647
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTAP1
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    BRDG1 (F-12) Alexa Fluor(r) 647
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 647
  • Gene ID26228
  • Target name
    STAP1
  • Target description
    signal transducing adaptor family member 1
  • Target synonyms
    BRDG1, STAP-1, signal-transducing adaptor protein 1, BCR downstream-signaling protein 1, docking protein BRDG1, stem cell adaptor protein 1
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAP-1/STAP1 using anti-STAP-1/STAP1 antibody (A09142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAP-1/STAP1 antigen affinity purified polyclonal antibody (Catalog # A09142-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAP-1/STAP1 at approximately 36 kDa. The expected band size for STAP-1/STAP1 is at 34 kDa.
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