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BRDT Antibody (aa948-960)

LS-C54592
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Rabbit, Rat
TargetBRDT
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BRDT Antibody (aa948-960)
  • Delivery Days Customer
    23
  • Applications
    ELISA
  • Applications Supplier
    Peptide-ELISA (1:32000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID676
  • Target name
    BRDT
  • Target description
    bromodomain testis associated
  • Target synonyms
    BRD6, CT9, SPGF21, bromodomain testis-specific protein, RING3-like protein, bromodomain, testis-specific, cancer/testis antigen 9
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BRDT using anti-BRDT antibody (A04985-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRDT antigen affinity purified polyclonal antibody (Catalog # A04985-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRDT at approximately 180 kDa. The expected band size for BRDT is at 108 kDa.
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BRDT antibody detects BRDT protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with BRDT antibody (GTX100201) at a dilution of 1:500.
Product group Antibodies
References
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