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Brn2 antibody

GTX114650
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Primate, Rat
TargetPOU3F2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Brn2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5454
  • Target name
    POU3F2
  • Target description
    POU class 3 homeobox 2
  • Target synonyms
    BRN2, N-Oct3, OCT7, OTF-7, OTF7, POUF3, brn-2, oct-7, POU domain, class 3, transcription factor 2, brain-2, brain-specific homeobox/POU domain protein 2, nervous system-specific octamer-binding transcription factor N-Oct-3, octamer-binding protein 7, octamer-binding transcription factor 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20265
  • Protein Name
    POU domain, class 3, transcription factor 2
  • Scientific Description
    POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]
  • Reactivity
    Human, Mouse, Primate, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

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  • Schaaf ZA, Tat L, Cannizzaro N, et al. WDFY3 mutation alters laminar position and morphology of cortical neurons. Mol Autism. 2022,13(1):27. doi: 10.1186/s13229-022-00508-3
    Read this paper
  • Soares MAF, Soares DS, Teixeira V, et al. Hierarchical reactivation of transcription during mitosis-to-G1 transition by Brn2 and Ascl1 in neural stem cells. Genes Dev. 2021,35(13-14):1020-1034. doi: 10.1101/gad.348174.120
    Read this paper
  • Yang CY, Hung YC, Cheng KH, et al. Loss of CC2D1A in Glutamatergic Neurons Results in Autistic-Like Features in Mice. Neurotherapeutics. 2021,18(3):2021-2039. doi: 10.1007/s13311-021-01072-z
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  • Wang J, Li T, Wang JL, et al. Talpid3-Mediated Centrosome Integrity Restrains Neural Progenitor Delamination to Sustain Neurogenesis by Stabilizing Adherens Junctions. Cell Rep. 2020,33(11):108495. doi: 10.1016/j.celrep.2020.108495
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  • Yamada S, Sato A, Sakakibara SI. Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex. iScience. 2020,23(5):101058. doi: 10.1016/j.isci.2020.101058
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  • Kitajima R, Nakai R, Imamura T, et al. Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells. Stem Cell Res. 2020,44:101749. doi: 10.1016/j.scr.2020.101749
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  • Yoon SJ, Elahi LS, Pașca AM, et al. Reliability of human cortical organoid generation. Nat Methods. 2019,16(1):75-78. doi: 10.1038/s41592-018-0255-0
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  • Wong WT, Matrone G, Tian X, et al. Discovery of novel determinants of endothelial lineage using chimeric heterokaryons. Elife. 2017,6:pii: e23588. doi: 10.7554/eLife.23588.
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  • Enriquez-Rios V, Dumitrache LC, Downing SM, et al. DNA-PKcs, ATM, and ATR Interplay Maintains Genome Integrity during Neurogenesis. J Neurosci. 2017,37(4):893-905. doi: 10.1523/JNEUROSCI.4213-15.2016
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