![Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX114650/GTX114650_40212_20160921_IHC-Fr_w_23060518_935.webp)
Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Brn2 antibody
GTX114650
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Primate, Rat
TargetPOU3F2
Overview
- SupplierGeneTex
- Product NameBrn2 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.2 mg/ml
- ConjugateUnconjugated
- Gene ID5454
- Target namePOU3F2
- Target descriptionPOU class 3 homeobox 2
- Target synonymsBRN2, N-Oct3, OCT7, OTF-7, OTF7, POUF3, brn-2, oct-7, POU domain, class 3, transcription factor 2, brain-2, brain-specific homeobox/POU domain protein 2, nervous system-specific octamer-binding transcription factor N-Oct-3, octamer-binding protein 7, octamer-binding transcription factor 7
- HostRabbit
- IsotypeIgG
- Protein IDP20265
- Protein NamePOU domain, class 3, transcription factor 2
- Scientific DescriptionPOU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]
- ReactivityHuman, Mouse, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:1000. Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Scale bar = 10 μm. Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:1000. Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114650/GTX114650_42732_20170412_IFA_w_23060518_619.webp)










![ELISA analysis of antigen using GTX60632 Brn2 antibody [6H12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60632/GTX60632_20170912_ELISA_w_23061123_910.webp)

