Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BRN3B Polyclonal Antibody, Unconjugated (bs-6985R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BRN3B Polyclonal Antibody, Unconjugated (bs-6985R) at 1:200 overnight at 4°C, DAB staining.
BRN3B Polyclonal Antibody
BS-6985R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetPOU4F2
Overview
- SupplierBioss
- Product NameBRN3B Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID5458
- Target namePOU4F2
- Target descriptionPOU class 4 homeobox 2
- Target synonymsBRN3.2, BRN3B, Brn-3b, POU domain, class 4, transcription factor 2, Brn3b POU domain transcription factor, POU domain protein, brain-3B, brain-specific homeobox/POU domain protein 3B
- HostRabbit
- IsotypeIgG
- Protein IDQ12837
- Protein NamePOU domain, class 4, transcription factor 2
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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