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Western Blot analysis of HeLa cells using Probetacellulin Polyclonal Antibody
Western Blot analysis of HeLa cells using Probetacellulin Polyclonal Antibody
Western Blot analysis of HeLa cells using Probetacellulin Polyclonal Antibody

BTC Antibody

CSB-PA003854
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetBTC
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Overview

  • Supplier
    Cusabio
  • Product Name
    BTC Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID685
  • Target name
    BTC
  • Target description
    betacellulin
  • Target synonyms
    probetacellulin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35070
  • Protein Name
    Probetacellulin
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BTC using anti-BTC antibody (A02171-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human BTC protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTC antigen affinity purified polyclonal antibody (Catalog # A02171-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BTC at approximately 15KD. The expected band size for BTC is at 9KD.
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