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BTC Monoclonal Antibody

BSM-60561M
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetBTC
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    BTC Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F2C9
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID685
  • Target name
    BTC
  • Target description
    betacellulin
  • Target synonyms
    probetacellulin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP35070
  • Protein Name
    Probetacellulin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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References
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Figure 1. Western blot analysis of BTC using anti-BTC antibody (A02171-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human BTC protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTC antigen affinity purified polyclonal antibody (Catalog # A02171-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BTC at approximately 15KD. The expected band size for BTC is at 9KD.
Product group Antibodies
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