C-HCV, Combined Hepatitis C Virus, NS3/NS4/NS5, recombinant, E. coli

Jena Bioscience

C-HCV, Combined Hepatitis C Virus, NS3/NS4/NS5, recombinant, E. coli

5

Western Blot, ELISA

Research Use Only

>95%

The protein contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions. Combined Hepatitis C Virus protein is purified by proprietary chromatographic techniques. Background: Sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then constructed into one recombinant antigen.

-20°C

12352202