![Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Kit (D816V Mutant) antibody [HL2653] (GTX639112) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Kit (D816V Mutant) antibody [HL2653] (GTX639112) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639112/GTX639112_45271_20240105_WB_B_24010821_755.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Kit (D816V Mutant) antibody [HL2653] (GTX639112) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
c-Kit (D816V Mutant) antibody [HL2653]
GTX639112
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetKIT
Overview
- SupplierGeneTex
- Product Namec-Kit (D816V Mutant) antibody [HL2653]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2653
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3815
- Target nameKIT
- Target descriptionKIT proto-oncogene, receptor tyrosine kinase
- Target synonymsC-Kit, CD117, MASTC, PBT, SCFR, mast/stem cell growth factor receptor Kit, c-Kit protooncogene, p145 c-kit, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, tyrosine-protein kinase Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
- HostRabbit
- IsotypeIgG
- Protein IDP10721
- Protein NameMast/stem cell growth factor receptor Kit
- Scientific DescriptionThis gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![c-Kit (D816V Mutant) antibody [HL2653] detects c-Kit (D816V Mutant) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% PFA. Green: c-Kit (D816V Mutant) stained by c-Kit (D816V Mutant) antibody [HL2653] (GTX639112) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). c-Kit (D816V Mutant) antibody [HL2653] detects c-Kit (D816V Mutant) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% PFA. Green: c-Kit (D816V Mutant) stained by c-Kit (D816V Mutant) antibody [HL2653] (GTX639112) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX639112/GTX639112_T-45194_20231229_ICC_IF_B_24011618_957.webp)




