
WB (peptide competition) analysis of M07e cells unstimulated (Lane 1) or stimulated with 250ng/mL SCF (Lane 2-5) using GTX25634 c-Kit (phospho Tyr823) antibody with non-phosphorylated peptide (Lane 3), generic phosphotyrosine-containing peptide (Lane 4), phosphopeptide immunogen (Lane 5) . The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
c-Kit (phospho Tyr823) antibody
GTX25634
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityAvian, Human, Mouse
TargetKIT
Overview
- SupplierGeneTex
- Product Namec-Kit (phospho Tyr823) antibody - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID3815
- Target nameKIT
- Target descriptionKIT proto-oncogene, receptor tyrosine kinase
- Target synonymsCD117; C-Kit; c-Kit protooncogene; mast/stem cell growth factor receptor Kit; MASTC; p145 c-kit; PBT; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
- HostRabbit
- IsotypeIgG
- Protein IDP10721
- Protein NameMast/stem cell growth factor receptor Kit
- Scientific DescriptionThis gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityAvian, Human, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203