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Western blot using GeneTexs affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42oC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45microm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500.
Western blot using GeneTexs affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42oC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45microm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500.
Western blot using GeneTexs affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42oC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45microm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500.

C-Myb antibody

GTX48705
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, Other Application
DownloadDatasheet
Product group Antibodies
ReactivityMonkey, Mouse
TargetMyb
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Overview

  • Supplier
    GeneTex
  • Product Name
    C-Myb antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2500. ELISA: 1:10000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.51 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID17863
  • Target name
    Myb
  • Target description
    myeloblastosis oncogene
  • Target synonyms
    AI550390; c-myb; gag-myb protein; M16449; myb proto-oncogene protein; myeloblastosis proto-oncogene product; proto-oncogene c-Myb; transcriptional activator Myb; tumor-specific myb protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06876
  • Protein Name
    Transcriptional activator Myb
  • Scientific Description
    The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb.
  • Reactivity
    Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203