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WB analysis of human B lymphoblast cells using GTX45636 C1ORF55 antibody at 0.2-1microg/ml.
WB analysis of human B lymphoblast cells using GTX45636 C1ORF55 antibody at 0.2-1microg/ml.
WB analysis of human B lymphoblast cells using GTX45636 C1ORF55 antibody at 0.2-1microg/ml.

C1ORF55 antibody, C-term

GTX45636
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSDE2
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Overview

  • Supplier
    GeneTex
  • Product Name
    C1ORF55 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID163859
  • Target name
    SDE2
  • Target description
    spliceosome associated SDE2
  • Target synonyms
    C1orf55, dJ671D7.1, splicing regulator SDE2, SDE2 telomere maintenance homolog, UPF0667 protein C1orf55, protein SDE2 homolog, replication stress response regulator SDE2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6IQ49
  • Protein Name
    Splicing regulator SDE2
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SDE2 using anti-SDE2 antibody (A15473-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDE2 antigen affinity purified polyclonal antibody (Catalog # A15473-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDE2 at approximately 54 kDa. The expected band size for SDE2 is at 50 kDa.
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