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ProductsBack/C3IP1 antibody [2G2]
WB analysis of HeLa cell lysate using GTX83228 KLHL12 antibody [2G2].
WB analysis of HeLa cell lysate using GTX83228 KLHL12 antibody [2G2].
WB analysis of HeLa cell lysate using GTX83228 KLHL12 antibody [2G2].

C3IP1 antibody [2G2]

GTX83228
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetKLHL12
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Overview

  • Supplier
    GeneTex
  • Product Name
    C3IP1 antibody [2G2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2G2
  • Conjugate
    Unconjugated
  • Gene ID59349
  • Target name
    KLHL12
  • Target description
    kelch like family member 12
  • Target synonyms
    C3IP1; CUL3-interacting protein 1; DKIR; DKIR homolog; kelch-like protein 12; kelch-like protein C3IP1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ53G59
  • Protein Name
    Kelch-like protein 12
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Control of the negative IRES trans-acting factor KHSRP by ubiquitination. Kung YA et al., 2017 Jan 9, Nucleic Acids Res
    Read more

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Figure 1. Western blot analysis of KLHL12 using anti-KLHL12 antibody (A08568-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLHL12 antigen affinity purified polyclonal antibody (Catalog # A08568-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLHL12 at approximately 63 kDa. The expected band size for KLHL12 is at 63 kDa.
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