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ProductsBack/C3IP1 (KLHL12) Mouse Monoclonal Antibody [Clone ID: 2G2]
Western blot analysis using KLHL12 mouse mAb against Hela (1) cell lysate.
Western blot analysis using KLHL12 mouse mAb against Hela (1) cell lysate.
Western blot analysis using KLHL12 mouse mAb against Hela (1) cell lysate.

C3IP1 (KLHL12) Mouse Monoclonal Antibody [Clone ID: 2G2]

AM06462SU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetKLHL12
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    C3IP1 (KLHL12) Mouse Monoclonal Antibody [Clone ID: 2G2]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2G2
  • Gene ID59349
  • Target name
    KLHL12
  • Target description
    kelch like family member 12
  • Target synonyms
    C3IP1, DKIR, kelch-like protein 12, CUL3-interacting protein 1, DKIR homolog, kelch-like protein C3IP1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ53G59
  • Protein Name
    Kelch-like protein 12
  • Scientific Description
    C3IP1 (KLHL12) mouse monoclonal antibody, clone 2G2, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KLHL12 using anti-KLHL12 antibody (A08568-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLHL12 antigen affinity purified polyclonal antibody (Catalog # A08568-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLHL12 at approximately 63 kDa. The expected band size for KLHL12 is at 63 kDa.
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