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C4orf49 / OSAP Antibody (aa176-204)

LS-C168150
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMGARP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    C4orf49 / OSAP Antibody (aa176-204)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:10 - 1:50), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84709
  • Target name
    MGARP
  • Target description
    mitochondria localized glutamic acid rich protein
  • Target synonyms
    C4orf49, CESP-1, HUMMR, OSAP, protein MGARP, corneal endothelium-specific protein 1, hypoxia up-regulated mitochondrial movement regulator protein, ovary-specific acidic protein
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C4orf49/MGARP using anti-C4orf49/MGARP antibody (A09345-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A2780 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C4orf49/MGARP antigen affinity purified polyclonal antibody (Catalog # A09345-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C4orf49/MGARP at approximately 38 kDa. The expected band size for C4orf49/MGARP is at 25 kDa.
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