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ProductsBack/C5a R1 antibody [10/92] (Biotin)

C5a R1 antibody [10/92] (Biotin)

GTX43902
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetC5ar1
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Overview

  • Supplier
    GeneTex
  • Product Name
    C5a R1 antibody [10/92] (Biotin)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: Neat. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10/92
  • Concentration
    0.1 mg/ml
  • Conjugate
    Biotin
  • Gene ID12273
  • Target name
    C5ar1
  • Target description
    complement component 5a receptor 1
  • Target synonyms
    C5aR, C5r1, Cd88, D7Msu1, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5a ligand, C5a-R, complement component 5, receptor 1
  • Host
    Rat
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C5a-R/C5ar1 using anti-C5a-R/C5ar1 antibody (A01898-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5a-R/C5ar1 antigen affinity purified polyclonal antibody (Catalog # A01898-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5a-R/C5ar1 at approximately 43KD. The expected band size for C5a-R/C5ar1 is at 43KD.
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