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Western blot All lanes: CA12 antibody at 2microg/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 39 kDa Observed band size: 40 kDa
Western blot All lanes: CA12 antibody at 2microg/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 39 kDa Observed band size: 40 kDa
Western blot All lanes: CA12 antibody at 2microg/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 39 kDa Observed band size: 40 kDa

CA12 Antibody

CSB-PA004367LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetCA12
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Overview

  • Supplier
    Cusabio
  • Product Name
    CA12 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID771
  • Target name
    CA12
  • Target description
    carbonic anhydrase 12
  • Target synonyms
    CA-XII, CAXII, HsT18816, T18816, carbonic anhydrase 12, carbonate dehydratase XII, carbonic anhydrase XII, carbonic dehydratase, tumor antigen HOM-RCC-3.1.3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43570
  • Protein Name
    Carbonic anhydrase 12
  • Scientific Description
    Reversible hydration of carbon dioxide.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CA12 using anti-CA12 antibody (A04063-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA12 antigen affinity purified polyclonal antibody (Catalog # A04063-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA12 at approximately 45 kDa. The expected band size for CA12 is at 39 kDa.
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