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Western Blot analysis of HepG2 cells using CA XIII Polyclonal Antibody
Western Blot analysis of HepG2 cells using CA XIII Polyclonal Antibody
Western Blot analysis of HepG2 cells using CA XIII Polyclonal Antibody

CA13 Antibody

CSB-PA001104
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetCA13
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Overview

  • Supplier
    Cusabio
  • Product Name
    CA13 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID377677
  • Target name
    CA13
  • Target description
    carbonic anhydrase 13
  • Target synonyms
    CAXIII, carbonic anhydrase 13, CA-XIII, carbonate dehydratase XIII, carbonic anhydrase XIII
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N1Q1
  • Protein Name
    Carbonic anhydrase 13
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Carbonic Anhydrase 13/CA13 using anti-Carbonic Anhydrase 13/CA13 antibody (A09186-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: rat intestines tissue lysates, Lane 7: mouse NTH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 13/CA13 antigen affinity purified polyclonal antibody (Catalog # A09186-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 13/CA13 at approximately 29KD. The expected band size for Carbonic Anhydrase 13/CA13 is at 29KD.
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