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Western blot All lanes: CA3 antibody at 2microg/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
Western blot All lanes: CA3 antibody at 2microg/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
Western blot All lanes: CA3 antibody at 2microg/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa

CA3 Antibody

CSB-PA004371LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    CA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID761
  • Target name
    CA3
  • Target description
    carbonic anhydrase 3
  • Target synonyms
    CAIII; CA-III; Car3; carbonate dehydratase III; carbonic anhydrase 3; carbonic anhydrase III, muscle specific; carbonic anhydrase IIII; epididymis secretory sperm binding protein Li 167mP; HEL-S-167mP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07451
  • Protein Name
    Carbonic anhydrase 3
  • Scientific Description
    Reversible hydration of carbon dioxide.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CA3 using anti-CA3 antibody (PB10049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, Lane 2: mouse cardiac muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA3 antigen affinity purified polyclonal antibody (Catalog # PB10049) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA3 at approximately 29 kDa. The expected band size for CA3 is at 30 kDa.
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Lane 1: Mouse Muscle tissue lysates; Lane 2: Rat Muscle tissue lysates probed with Carbonic anhydrase 3 Polyclonal Antibody, Unconjugated (bs-24471R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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