CALB1 Calbindin-1 Rat Recombinant Protein

Boster Bio

CALB1 Calbindin-1 Rat Recombinant Protein

9

ImmunoHistoChemistry, RadioImmunoAssay

Research Use Only

Calbindin

Recombinant Rat Calbindin-1 produced in E. coli. The Rat CALB1 is purified by proprietary chromatographic techniques. Applications: CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption, we suggest the following procedure: A- Dilute 1 microl of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5000). B- Add 1 microg of the recombinant protein to 1 ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

E.coli

-20°C,2°C to 8°C,RT

12352202