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Calcium Sensing Receptor Antibody

BS-8524R
Bioss Antibodies
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetCASR
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Calcium Sensing Receptor Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID846
  • Target name
    CASR
  • Target description
    calcium sensing receptor
  • Target synonyms
    CAR; EIG8; extracellular calcium-sensing receptor; FHH; FIH; GPRC2A; hCasR; HHC; HHC1; HYPOC1; NSHPT; parathyroid Ca(2+)-sensing receptor 1; parathyroid cell calcium-sensing receptor 1; PCAR1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41180
  • Protein Name
    Extracellular calcium-sensing receptor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Involvement of calcium-sensing receptor activation in the alleviation of intestinal inflammation in a piglet model by dietary aromatic amino acid supplementation. Liu H et al., 2018 Dec, Br J Nutr
    Read more

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Figure 1. Western blot analysis of CASR using anti-CASR antibody (PB9924). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASR antigen affinity purified polyclonal antibody (Catalog # PB9924) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASR at approximately 150 kDa. The expected band size for CASR is at 121 kDa.
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