WB analysis of unphosphorylated and phosphorylated rat brain homogenate (left and right lanes respectively) using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1] or GTX22725CaMKII alpha antibody [6G9] .
![ICC/IF analysis of Neuro-2a cells (left panel) or cells stimulated with Lamda-Phosphatase (Lambda-PPase) (right panel) using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute](https://www.genetex.com/upload/website/prouct_img/normal/GTX22724/GTX22724_381_ICC-IF_w_23060620_151.webp "ICC/IF analysis of Neuro-2a cells (left panel) or cells stimulated with Lamda-Phosphatase (Lambda-PPase) (right panel) using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute")
![ICC/IF analysis of U251 cells using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22724/GTX22724_382_ICC-IF_w_23060620_347.webp "ICC/IF analysis of U251 cells using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
WB analysis of unphosphorylated and phosphorylated rat brain homogenate (left and right lanes respectively) using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1] or GTX22725CaMKII alpha antibody [6G9] .
CaMKII alpha (phospho Thr286) antibody [22B1]
GTX22724
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rabbit, Rat
TargetCamk2a
Overview
- SupplierGeneTex
- Product NameCaMKII alpha (phospho Thr286) antibody [22B1] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody has been shown to detect the phosphorylated alpha subunit CaM kinase II only. It does not detect the non-phosphorylated alpha subunit CaM kinase II nor with the ~60 kDa beta subunit in either phosphorylation state. Immunofluorescence stainin
- Application Supplier NoteWB: 1:2,000. ICC/IF: 1:50-1:100. FACS: 1microg per 106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID22B1
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID25400
- Target nameCamk2a
- Target descriptioncalcium/calmodulin-dependent protein kinase II alpha
- Target synonymsalpha CaM kinase II; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II subunit alpha; caM kinase II subunit alpha; caMK-II subunit alpha; caM-kinase II alpha chain; PK2CDD; PKCCD
- HostMouse
- IsotypeIgG1
- Protein IDP11275
- Protein NameCalcium/calmodulin-dependent protein kinase type II subunit alpha
- ReactivityHuman, Mouse, Rabbit, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Association of CaMK2A and MeCP2 signaling pathways with cognitive ability in adolescents. Lee LC et al., 2021 Oct 4, Mol BrainRead more