Boiled and unboiled Jurkat whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640596/GTX640596_T-45460_20240705_WB_B_ub_24070822_341.webp "Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) unboiled Jurkat whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640596/GTX640596_45537_20241025_WB_shRNA_watermark_24103022_857.webp "Non-transfected (–) and transfected (+) unboiled Jurkat whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Cannabinoid receptor 2 antibody [HL3120] detects Cannabinoid receptor 2 protein by immunofluorescent analysis. Sample: Jurkat cells were fixed in ice-cold MeOH for 5 min. Green: Cannabinoid receptor 2 stained by Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640596/GTX640596_T-45460_20240816_ICC_IF_24110700_813.webp "Cannabinoid receptor 2 antibody [HL3120] detects Cannabinoid receptor 2 protein by immunofluorescent analysis. Sample: Jurkat cells were fixed in ice-cold MeOH for 5 min. Green: Cannabinoid receptor 2 stained by Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Boiled and unboiled Jurkat whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Cannabinoid receptor 2 antibody [HL3120]
GTX640596
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCNR2
Overview
- SupplierGeneTex
- Product NameCannabinoid receptor 2 antibody [HL3120]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3120
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID1269
- Target nameCNR2
- Target descriptioncannabinoid receptor 2
- Target synonymsCB-2, CB2, CX5, cannabinoid receptor 2, cannabinoid receptor 2 (macrophage)
- HostRabbit
- IsotypeIgG
- Protein IDP34972
- Protein NameCannabinoid receptor 2
- Scientific DescriptionThe cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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