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Western Blot Positive WB detected in: HepG2 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate All lanes: CAP1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate All lanes: CAP1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate All lanes: CAP1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa

CAP1 Antibody

CSB-PA004486LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCAP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CAP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10487
  • Target name
    CAP1
  • Target description
    cyclase associated actin cytoskeleton regulatory protein 1
  • Target synonyms
    adenylate cyclase associated protein 1; adenylyl cyclase-associated protein 1; CAP; CAP, adenylate cyclase-associated protein 1; CAP1-PEN; testis secretory sperm-binding protein Li 218p
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01518
  • Protein Name
    Adenylyl cyclase-associated protein 1
  • Scientific Description
    Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CAP1 using anti-CAP1 antibody (PB9923). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: SMMC7721 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAP1 antigen affinity purified polyclonal antibody (Catalog # PB9923) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAP1 at approximately 60 kDa. The expected band size for CAP1 is at 52 kDa.
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