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ProductsBack/CAPN1 Recombinant Antibody, Biotin Conjugated

CAPN1 Recombinant Antibody, Biotin Conjugated

BSM-61500R-BIOTIN
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCAPN1
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Overview

  • Supplier
    Bioss
  • Product Name
    CAPN1 Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID823
  • Target name
    CAPN1
  • Target description
    calpain 1
  • Target synonyms
    CANP, CANP1, CANPL1, SPG76, muCANP, muCL, calpain-1 catalytic subunit, CANP 1, calcium-activated neutral proteinase 1, calpain 1, (mu/I) large subunit, calpain mu-type, calpain, large polypeptide L1, calpain-1 large subunit, cell proliferation-inducing gene 30 protein, cell proliferation-inducing protein 30, micromolar-calpain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07384
  • Protein Name
    Calpain-1 catalytic subunit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calpain 1 using anti-Calpain 1 antibody (PB9330). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human T47D whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpain 1 antigen affinity purified polyclonal antibody (Catalog # PB9330) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpain 1 at approximately 82 kDa. The expected band size for Calpain 1 is at 82 kDa.
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