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CAPN5 / Calpain 5 Antibody (aa1-381)

LS-C185533
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCAPN5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CAPN5 / Calpain 5 Antibody (aa1-381)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:100 - 1:1000), WB (1:500 - 1:3000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.39 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID726
  • Target name
    CAPN5
  • Target description
    calpain 5
  • Target synonyms
    ADNIV; calpain htra-3; calpain-5; HTRA3; nCL-3; new calpain 3; testis tissue sperm-binding protein Li 91mP; VRNI
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CAPN5 using anti-CAPN5 antibody (A08350-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPN5 antigen affinity purified polyclonal antibody (Catalog # A08350-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAPN5 at approximately 73 kDa. The expected band size for CAPN5 is at 73 kDa.
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