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ProductsBack/Calpain 5 antibody [N1C1]

Calpain 5 antibody [N1C1]

GTX103264
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetCAPN5
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Overview

  • Supplier
    GeneTex
  • Product Name
    Calpain 5 antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.39 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID726
  • Target name
    CAPN5
  • Target description
    calpain 5
  • Target synonyms
    ADNIV; calpain htra-3; calpain-5; HTRA3; nCL-3; new calpain 3; testis tissue sperm-binding protein Li 91mP; VRNI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15484
  • Protein Name
    Calpain-5
  • Scientific Description
    Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Impaired activity and membrane association of most calpain-5 mutants causal for neovascular inflammatory vitreoretinopathy.
    Read more
  • The C2 domain of calpain 5 contributes to enzyme activation and membrane localization. Bondada V et al., 2021 Jun, Biochim Biophys Acta Mol Cell Res
    Read more
  • Characterization of mitochondrial calpain-5. Chukai Y et al., 2021 Apr, Biochim Biophys Acta Mol Cell Res
    Read more
  • A novel de novo CAPN5 mutation in a patient with inflammatory vitreoretinopathy, hearing loss, and developmental delay. Velez G et al., 2018 Jun, Cold Spring Harb Mol Case Stud
    Read more
  • Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses. Schaefer KA et al., 2016 May 1, Invest Ophthalmol Vis Sci
    Read more

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Figure 1. Western blot analysis of CAPN5 using anti-CAPN5 antibody (A08350-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPN5 antigen affinity purified polyclonal antibody (Catalog # A08350-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAPN5 at approximately 73 kDa. The expected band size for CAPN5 is at 73 kDa.
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