Bio-Connect
WB analysis of mouse lung tissue lysate (35ug/lane) using GTX81426 Carbonic anhydrase IV antibody, C-term.
WB analysis of mouse lung tissue lysate (35ug/lane) using GTX81426 Carbonic anhydrase IV antibody, C-term.
WB analysis of mouse lung tissue lysate (35ug/lane) using GTX81426 Carbonic anhydrase IV antibody, C-term.

Carbonic anhydrase IV antibody, C-term

GTX81426
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCA4
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    Carbonic anhydrase IV antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID762
  • Target name
    CA4
  • Target description
    carbonic anhydrase 4
  • Target synonyms
    CAIV; CA-IV; Car4; carbonate dehydratase IV; carbonic anhydrase 4; carbonic anhydrase IV; carbonic dehydratase IV; RP17
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22748
  • Protein Name
    Carbonic anhydrase 4
  • Scientific Description
    Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCA4
  • SizePrice
Immunohistochemistry analysis in human rectum and lymph node tissues using HPA011089 antibody. Corresponding CA4 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoHistoChemistry
TargetCA4
  • SizePrice
IHC image of CSB-PA004372LA01HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
CA4 AntibodyCSB-PA004372LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetCA4
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetCA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCA4
  • SizePrice
Anti-CA4 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Figure 1. Western blot analysis of CA IV using anti-CA IV antibody (A01523-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA IV antigen affinity purified polyclonal antibody (Catalog # A01523-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA IV at approximately 35KD. The expected band size for CA IV is at 35KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetCA4
  • SizePrice