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Carboxypeptidase O / CPO Antibody

LS-C680316
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetCPO
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Carboxypeptidase O / CPO Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID130749
  • Target name
    CPO
  • Target description
    carboxypeptidase O
  • Target synonyms
    carboxypeptidase O; metallocarboxypeptidase C; metallocarboxypeptidase O
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunofluorescence staining of HepG2 cells with CSB-PA005909LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of CPO using anti-CPO antibody (A03649-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human AGS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPO antigen affinity purified polyclonal antibody (Catalog # A03649-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPO at approximately 57 kDa. The expected band size for CPO is at 43 kDa.
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