Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Bakers Yeast) are shown to detect under reducing conditions of SDS-PAGE the 61,000 dalton enzyme in cellular extracts. Approximately 10 microg of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-anti-Rabbit IgG (H&L) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Bakers Yeast) are shown to detect under reducing conditions of SDS-PAGE the 61,000 dalton enzyme in cellular extracts. Approximately 10 microg of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-anti-Rabbit IgG (H&L) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
Carboxypeptidase Y antibody
GTX48866
Overview
- SupplierGeneTex
- Product NameCarboxypeptidase Y antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ELISA: 1:5000-1:25000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration90 mg/ml
- ConjugateUnconjugated
- Gene ID855343
- Target namePRC1
- Target descriptioncarboxypeptidase C PRC1
- Target synonymscarboxypeptidase C PRC1; CPY1; LBC1; YMR297W
- HostRabbit
- IsotypeIgG
- Protein IDP00729
- Protein NameCarboxypeptidase Y
- ReactivityYeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203