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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA233585(CARD9 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA233585(CARD9 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA233585(CARD9 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200)

CARD9 Antibody

CSB-PA233585
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCARD9
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Overview

  • Supplier
    Cusabio
  • Product Name
    CARD9 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64170
  • Target name
    CARD9
  • Target description
    caspase recruitment domain family member 9
  • Target synonyms
    CANDF2, IMD103, hCARD9, caspase recruitment domain-containing protein 9, caspase recruitment domain protein 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H257
  • Protein Name
    Caspase recruitment domain-containing protein 9
  • Scientific Description
    The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CARD9 using anti-CARD9 antibody (A01410-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD9 antigen affinity purified polyclonal antibody (Catalog # A01410-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD9 at approximately 63 kDa. The expected band size for CARD9 is at 62 kDa.
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Immunohistochemistry analysis in human spleen and cerebral cortex tissues using HPA059502 antibody. Corresponding CARD9 RNA-seq data are presented for the same tissues.
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