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ProductsBack/Cardiac Troponin I/TNNC1 Polyclonal Antibody
Formalin-fixed and paraffin embedded rabbit heart labeled with Anti CTn1/Troponin I/TNNC1 Polyclonal Antibody,Unconjugated (bs-0799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rabbit heart labeled with Anti CTn1/Troponin I/TNNC1 Polyclonal Antibody,Unconjugated (bs-0799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rabbit heart labeled with Anti CTn1/Troponin I/TNNC1 Polyclonal Antibody,Unconjugated (bs-0799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Cardiac Troponin I/TNNC1 Polyclonal Antibody

BS-0799R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTNNI3
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Overview

  • Supplier
    Bioss
  • Product Name
    Cardiac Troponin I/TNNC1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7137
  • Target name
    TNNI3
  • Target description
    troponin I3, cardiac type
  • Target synonyms
    CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle, cardiomyopathy, dilated 2A (autosomal recessive), troponin I type 3 (cardiac)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19429
  • Protein Name
    Troponin I, cardiac muscle
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Kim CW, Go RE, Ko EB, et al. Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Environ Toxicol. 2020,35(1):66-77. doi: 10.1002/tox.22843
    Read this paper
  • Ola-Davies OE, Oyagbemi AA, Omobowale TO, et al. Ameliorative effects of Annona muricata Linn. (Annonaceae) against potassium dichromate-induced hypertension in vivo: involvement of Kim-1/p38 MAPK/Nrf2 signaling. J Basic Clin Physiol Pharmacol. 2019,30(4). doi: 10.1515/jbcpp-2018-0172
    Read this paper
  • Oyagbemi AA, Omobowale TO, Ola-Davies OE, et al. Ameliorative effect of Rutin on sodium fluoride-induced hypertension through modulation of Kim-1/NF-κB/Nrf2 signaling pathway in rats. Environ Toxicol. 2018,33(12):1284-1297. doi: 10.1002/tox.22636
    Read this paper
  • Ruan Z, Zhu L, Yin Y, et al. Overexpressing NKx2.5 increases the differentiation of human umbilical cord drived mesenchymal stem cells into cardiomyocyte-like cells. Biomed Pharmacother. 2016,78:110-115. doi: 10.1016/j.biopha.2016.01.020
    Read this paper
  • Ruan ZB, Zhu L, Yin YG, et al. Inhibitor of p53-p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells. Cytotechnology. 2016,68(4):1257-65. doi: 10.1007/s10616-015-9886-5
    Read this paper
  • Zhu L, Ruan Z, Yin Y, et al. Expression and significance of DLL4--Notch signaling pathway in the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyocytes induced by 5-azacytidine. Cell Biochem Biophys. 2015,71(1):249-53. doi: 10.1007/s12013-014-0191-2
    Read this paper
  • Gao LR, Zhang NK, Ding QA, et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. Cell Transplant. 2013,22(10):1883-900. doi: 10.3727/096368912X662444
    Read this paper

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Figure 1. Western blot analysis of Cardiac Troponin I/TNNI3 using anti-Cardiac Troponin I/TNNI3 antibody (A01720-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin I/TNNI3 antigen affinity purified polyclonal antibody (Catalog # A01720-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin I/TNNI3 at approximately 24 kDa. The expected band size for Cardiac Troponin I/TNNI3 is at 24 kDa.
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