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Formalin-fixed and paraffin embedded rabbit heart labeled with Anti CTn1/Troponin I/TNNC1 Polyclonal Antibody,Unconjugated (bs-0799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rabbit heart labeled with Anti CTn1/Troponin I/TNNC1 Polyclonal Antibody,Unconjugated (bs-0799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rabbit heart labeled with Anti CTn1/Troponin I/TNNC1 Polyclonal Antibody,Unconjugated (bs-0799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Cardiac Troponin I/TNNC1 Polyclonal Antibody

BS-0799R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTNNI3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Cardiac Troponin I Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7137
  • Target name
    TNNI3
  • Target description
    troponin I3, cardiac type
  • Target synonyms
    cardiomyopathy, dilated 2A (autosomal recessive); CMD1FF; CMD2A; CMH7; cTnI; RCM1; TNNC1; troponin I type 3 (cardiac); troponin I, cardiac muscle
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19429
  • Protein Name
    Troponin I, cardiac muscle
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Kim CW et al., 2020 Jan, Environ Toxicol
    Read more
  • Ameliorative effects of Annona muricata Linn. (Annonaceae) against potassium dichromate-induced hypertension in vivo: involvement of Kim-1/p38 MAPK/Nrf2 signaling. Ola-Davies OE et al., 2019 May 3, J Basic Clin Physiol Pharmacol
    Read more
  • Ameliorative effect of Rutin on sodium fluoride-induced hypertension through modulation of Kim-1/NF-kappaB/Nrf2 signaling pathway in rats. Oyagbemi AA et al., 2018 Dec, Environ Toxicol
    Read more
  • Overexpressing NKx2.5 increases the differentiation of human umbilical cord drived mesenchymal stem cells into cardiomyocyte-like cells. Ruan Z et al., 2016 Mar, Biomed Pharmacother
    Read more
  • Inhibitor of p53-p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells. Ruan ZB et al., 2016 Aug, Cytotechnology
    Read more
  • Expression and significance of DLL4--Notch signaling pathway in the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyocytes induced by 5-azacytidine. Zhu L et al., 2015 Jan, Cell Biochem Biophys
    Read more
  • Common expression of stemness molecular markers and early cardiac transcription factors in human Whartons jelly-derived mesenchymal stem cells and embryonic stem cells. Gao LR et al., 2013, Cell Transplant
    Read more

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Figure 1. Western blot analysis of Cardiac Troponin I/TNNI3 using anti-Cardiac Troponin I/TNNI3 antibody (A01720-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin I/TNNI3 antigen affinity purified polyclonal antibody (Catalog # A01720-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin I/TNNI3 at approximately 24 kDa. The expected band size for Cardiac Troponin I/TNNI3 is at 24 kDa.
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