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Cardiac Troponin T antibody [1C11]

Cardiac Troponin T antibody [1C11]

GTX28295
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application, Purification/Extraction/Isolation
Product group Antibodies
TargetTNNT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cardiac Troponin T antibody [1C11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application, Purification/Extraction/Isolation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1C11
  • Conjugate
    Unconjugated
  • Gene ID7139
  • Target name
    TNNT2
  • Target description
    troponin T2, cardiac type
  • Target synonyms
    cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TnTC; troponin T type 2 (cardiac); troponin T, cardiac muscle; truncated cardiac troponin T
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP45379
  • Protein Name
    Troponin T, cardiac muscle
  • Scientific Description
    The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging.
    Read more
  • Enhancement of pacing function by HCN4 overexpression in human pluripotent stem cell-derived cardiomyocytes. Saito Y et al., 2022 Apr 1, Stem Cell Res Ther
    Read more
  • Convergent synthesis of diversified reversible network leads to liquid metal-containing conductive hydrogel adhesives. Xu Y et al., 2021 Apr 23, Nat Commun
    Read more
  • Beating Heart Cells from Hair-Follicle-Associated Pluripotent (HAP) Stem Cells. Hoffman RM et al., 2018, Methods Mol Biol
    Read more
  • Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells. Hoffman RM, 2019, Methods Mol Biol
    Read more
  • HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction. Williams AL et al., 2018 Jul 1, Physiol Genomics
    Read more
  • A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells. Lao S et al., 2017 Jul, Mol Med Rep
    Read more
  • Hypoxia Enhances Differentiation of Hair Follicle-Associated-Pluripotent (HAP) Stem Cells to Cardiac-Muscle Cells. Shirai K et al., 2017 Mar, J Cell Biochem
    Read more
  • Protocols for Efficient Differentiation of Hair Follicle-Associated Pluripotent (HAP) Stem Cells to Beating Cardiac Muscle Cells. Yashiro M et al., 2016, Methods Mol Biol
    Read more
  • Early-age-dependent selective decrease of differentiation potential of hair-follicle-associated pluripotent (HAP) stem cells to beating cardiac-muscle cells. Yamazaki A et al., 2016 Oct, Cell Cycle
    Read more

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Figure 1. Western blot analysis of Cardiac Troponin T using anti-Cardiac Troponin T antibody (A01154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin T antigen affinity purified polyclonal antibody (Catalog # A01154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin T at approximately 43KD. The expected band size for Cardiac Troponin T is at 36KD.
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