More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CARS antibody
WB analysis of various sample lysates using GTX32489 CARS antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32489 CARS antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32489 CARS antibody. Dilution : 1:1000 Loading : 25microg per lane

CARS antibody

GTX32489
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCARS1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    CARS antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:10 - 1:100. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID833
  • Target name
    CARS1
  • Target description
    cysteinyl-tRNA synthetase 1
  • Target synonyms
    CARS; CYSRS; cysteine translase; cysteine tRNA ligase 1, cytoplasmic; cysteine--tRNA ligase, cytoplasmic; MCDDBH; MDBH; MGC:11246
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49589
  • Protein Name
    Cysteine--tRNA ligase, cytoplasmic
  • Scientific Description
    This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
Cars Polyclonal AntibodyCAC08283
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCARS1
  • SizePrice
Product group Antibodies
Assay Genie
CARS Monoclonal AntibodyCAB0438
TargetCARS1
  • SizePrice
Product group Antibodies
Bioss Antibodies
CARS1 Recombinant AntibodyBSM-62444R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCARS1
  • SizePrice
Product group Antibodies
MedChem Express
CARS AntibodyHY-P80584
TargetCARS1
  • SizePrice
Western Blot Positive WB detected in: PC-3 whole cell lysate, Mouse lung tissue, Mouse kidney tissue All lanes: CARS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 83, 95 kDa Observed band size: 86 kDa
Product group Antibodies
Cusabio
CARS AntibodyCSB-PA004529LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCARS1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-CARS Antibody, Affinity PurifiedA302-409A
ApplicationsImmunoPrecipitation, Western Blot
TargetCARS1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CARS Antibody144-06540
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetCARS1
  • SizePrice
Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using AMAb90971 antibody. Corresponding CARS RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-CARS AntibodyAMAB90971
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetCARS1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CARS1 / CARS AntibodyLS-C748712
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetCARS1
  • SizePrice
Figure 1. Western blot analysis of CARS using anti-CARS antibody (A00259-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysates, Lane 2: human COLO-320 cell lysates, Lane 3: human A549 cell lysates, Lane 4: human PANC-1 cell lysates, Lane 5: human 22RV1 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARS antigen affinity purified polyclonal antibody (Catalog # A00259-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARS at approximately 85KD. The expected band size for CARS is at 85KD.
Product group Antibodies
Boster Bio
Anti-CARS Antibody Picoband(r)A00259-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCARS1
  • SizePrice