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Western Blot Positive WB detected in: PC-3 whole cell lysate, Mouse lung tissue, Mouse kidney tissue All lanes: CARS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 83, 95 kDa Observed band size: 86 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate, Mouse lung tissue, Mouse kidney tissue All lanes: CARS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 83, 95 kDa Observed band size: 86 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate, Mouse lung tissue, Mouse kidney tissue All lanes: CARS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 83, 95 kDa Observed band size: 86 kDa

CARS Antibody

CSB-PA004529LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCARS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CARS Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID833
  • Target name
    CARS1
  • Target description
    cysteinyl-tRNA synthetase 1
  • Target synonyms
    CARS; CYSRS; cysteine translase; cysteine tRNA ligase 1, cytoplasmic; cysteine--tRNA ligase, cytoplasmic; MCDDBH; MDBH; MGC:11246
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49589
  • Protein Name
    Cysteine--tRNA ligase, cytoplasmic
  • Scientific Description
    cytoplasm, cytosol, ATP binding, cysteine-tRNA ligase activity, protein homodimerization activity, tRNA binding, cysteinyl-tRNA aminoacylation, gene expression, tRNA aminoacylation for protein translation
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CARS using anti-CARS antibody (A00259-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysates, Lane 2: human COLO-320 cell lysates, Lane 3: human A549 cell lysates, Lane 4: human PANC-1 cell lysates, Lane 5: human 22RV1 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARS antigen affinity purified polyclonal antibody (Catalog # A00259-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARS at approximately 85KD. The expected band size for CARS is at 85KD.
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